Optimize qPCR Using SYBR Green Assays - Ask TaqMan #38
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Optimize qPCR Using SYBR Green Assays - Ask TaqMan #38
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Anjali Rana on Instagram: "Today we’re learning how to set up a qPCR reaction using Bio-Rad’s SYBR Green Supermix. qPCR (quantitative PCR) helps you measure gene expression or DNA quantity in real time - but only if your reaction is set up correctly 👇 🧪 qPCR MASTER MIX for 10 µl reaction: 5 µl SYBR Green iTaq Supermix 2 µl nuclease-free water 0.5 µl forward primer 0.5 µl reverse primer 2 µl DNA / cDNA ✨ Always protect SYBR Green from light, it’s light sensitive 💡 Save this reel for your next
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